RBRC10950, C57BL/6-Tg(CAG-Sphk1)H1Ytkw

RBRC No. RBRC10950
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG-Sphk1)H1Ytkw
Former Common name Sphk1 transgenic mice (Tg line: High)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol Sphk1
Symbol name sphingosine kinase 1
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cardiovasc Res. 2010 Feb 1;85(3):484-93. doi: 10.1093/cvr/cvp312
Additional information
1 No Data
Depositor Takuwa, Yoh (Kanazawa University Graduate School of Medical Science) Takuwa, Yoh
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10963, B6.129-Tg(Nanog-RFP)2Szki

RBRC No. RBRC10963
Type TransgeneCartagena
Species Mus musculus
Strain name B6.129-Tg(Nanog-RFP)2Szki
Former Common name Nanog-RFP Tg mouse
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Nanog promoter
2 Symbol RFP
Symbol name Discosoma sp. Red Fluorescent Protein
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis, 57, e23334 (2019).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Suzuki, Atsushi (Medical institute of bioregulation, Kyushu University) Suzuki, Atsushi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Live
Live mouse :

RBRC06200, B6C3-Tg(Nodal-lacZ)2yHmd

RBRC No. RBRC06200
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3-Tg(Nodal-lacZ)2yHmd
Former Common name Nodal-lacZ/BAC(2y), Nodal-lacZ Tg#2y
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuyoshi Takaoka, Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain description Nodal-lacZ transgenic mice. The transgene may be integrated into chromosome Y.
Colony maintenance Carrier (male) x Noncarrier (female) [or Crossing to Jcl:B6C3F1]
The transgene may be integrated into chromosome Y.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome Y
Common name No Data
Symbol description No Data
Promoter mouse Nodal promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome Y
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06216, B6.Cg-Mc4r<tm1.2Hko>

RBRC No. RBRC06216
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Mc4r<tm1.2Hko>
Former Common name MCR Delta FRT/+ (GWAS023), B6-Mc4r floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description MCR4 (Mcr4) gene floxed mice. Exon 1 was flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol Mc4r
Symbol name melanocortin 4 receptor
Chromosome 18
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 18
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06234, B6.DS-Trpv3<Nh>/Shi

RBRC No. RBRC06234
Type Spontaneous Mutation
Species Mus musculus
Strain name B6.DS-Trpv3<Nh>/Shi
Former Common name B6-Nh
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shionogi & Co., Ltd.. Arose in DS mouse that has been established as an inbred strain from a dd closed colony mice.
Strain description Non-hair dominant spontaneous mutant mice arose in DS/Shi inbred souse strain. A G1717A point mutation has been found in the Trpv3, which results in Gly 573 being replaced with Ser. Coisogenic strain (RBRC06233), C57BL/6J (C57BL/6JShi) congenic (RBRC06234) and BALB/c (BALB/cMsShi) congenic (RBRC06235).
Colony maintenance Wild-type (female) x Heterozygote (male)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Trpv3
Symbol name transient receptor potential cation channel, subfamily V, member 3
Chromosome 11
Common name No Data
Symbol description No Data
References






Research applications Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 2) The RECIPIENT must inform the DEPOSITOR the research plan using the BIOLOGICAL RESOURCE and must obtain a prior written consent form the DEPOSITOR using the APPROVAL FORM specified by the DEPOSITOR. 3) The RECIPIENT shall use the BIOLOGICAL RESOURCE exclusively for the purpose of research which was written in the research plan. 4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must inform the DEPOSITOR of the contents of such publication in advance. 5) The RECIPIENT contact and discuss with the DEPOSITOR in case of filing a patent application based on research results by using the BIOLOGICAL RESOURCES. 6) The RECIPIENT must contact and discuss with the DEPOSITOR in case of application for any commercial use.
Additional information
1 No Data
Depositor Yasui, Kiyoshi (Shionogi & Co., Ltd.) Yasui, Kiyoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06258, C57BL/6-Tg(Cacng5-EGFP)500Bsi

RBRC No. RBRC06258
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Cacng5-EGFP)500Bsi
Former Common name Cacng5 promoter-GFP, B6-Cacng5-GFP Tg#500
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoshiaki Shinohara and Hajime Hirose, RIKEN Brain Science Institute. The BAC transgenic construct containing the mouse Cacng5 gene with IRES-GFP (GENSAT1-BX1300) was used. C57BL/6 background.
Strain description hese transgenic mice express IRES-GFP under the control of the mouse Cacng5 promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Eur. J. Neurosci., 35, 702-710 (2012 ).
The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the GENSAT Project (GENSAT1-BX1300) as the source of the original BAC transgenic construct are requested.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Hirase, Hajime (RIKEN) Hirase, Hajime
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06296, CAJcl.Cg-Aicda<tm2.1Hon>

RBRC No. RBRC06296
Type Targeted MutationCartagena
Species Mus musculus
Strain name CAJcl.Cg-Aicda<tm2.1Hon>
Former Common name BALBcAJcl-AID G23S, BALBcA-AID/G23S KI
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. Bruce 4 ES cells were used. The mutant mice were backcrossed to BALB/cAJcl.
Strain description Aicda G23S mutant knock-in mice. BALB/cAJcl background. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology).
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Aicda
Symbol name activation-induced cytidine deaminase
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06316, C57BL/6N-Sst<tm1b(KOMP)Wtsi>/F04

RBRC No. RBRC06316
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Sst<tm1b(KOMP)Wtsi>/F04
Former Common name No Data
H-2 Haplotype No Data
ES cell line JM8A3.N1 [C57BL/6N-A]
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
Additional information
1 No Data
Depositor KOMP, The Knockout Mouse Project (University of California, Davis) KOMP, The Knockout Mouse Project
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06333, C57BL/6N-Sept5<tm1b(EUCOMM)Hmgu>/B10

RBRC No. RBRC06333
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Sept5<tm1b(EUCOMM)Hmgu>/B10
Former Common name No Data
H-2 Haplotype No Data
ES cell line JM8A3.N1 [C57BL/6N-A]
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Sept5
Symbol name septin 5
Chromosome 16
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06350, B6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz

RBRC No. RBRC06350
Type Gene Trap
Species Mus musculus
Strain name B6.129S2-Asf1b<Gt(ROSABetageo)437Muv>/Skmz
Former Common name B6-CIA-II, B6-Asf1b Gt
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuhisa Sekimizu, Graduate School of Pharmaceutical Sciences in 2007. CIA-II (RBRC06350): Originated from Asf1b<Gt(ROSABetageo)437Muv> gene trapped mice (EMMA ID:01154). Backcrossed to C57BL/6. CIA-I (RBRC06351 and RBRC06352): Generated by using Asf1a<Gt(S7-4B1)Sor> gene trapped ES cells (MMRRC #13934-USD/S7-4B1). The mutant mice were backcrossed to ICR and C57BL/6 mice, respectively.
Strain description CIA-II(Asf1b) gene trapped mice (B6 background)(RBRC06350), CIA-I (Asf1a) gene trapped mice (ICR mixed background: RBRC06351 and B6 background: RBRC06352). Homozygous and heterozygous mutant mice for CIA-II gene show no obvious abnormality. Homozygous mutant mice for CIA-I of ICR mixed and C57BL/6 background were perinatal and embryonic (around E12.5) lethal, respectively.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Asf1b
Symbol name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol LTR
Symbol name retrovirus LTR (viral long terminal repeat)
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 8
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
References Genes Dev. 1991 Sep;5(9):1513-23.

Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。 (will be announced).
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Sekimizu, Kazuhisa (The University of Tokyo) Sekimizu, Kazuhisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months