RBRC00067, C.Cg-Tnc<tm1Sia>/Rbrc

RBRC No. RBRC00067
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name C.Cg-Tnc<tm1Sia>/Rbrc
Former Common name BALB/cA-TgH(Tnc), BALB/c-TN, TNKO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain BALB/cAJcl
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. M. Kusakabe. Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN in 1990’s. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to BALB/cA.
Strain description C.B6CB-Tnc<tm1Sia>. Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the feild of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction, etc. The tenascin C deficient congenic strains were developed successive by backcrossing into A (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenance Homozygote x Homozygote
Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Tnc
Symbol name tenascin C
Chromosome 4
Common name Tnc; tenascin C, Hxb, Ten, TN-C, AI528729
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 4
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Am J Pathol. 1998 May;152(5):1237-45.
Biochem Biophys Res Commun. 1996 Apr 5;221(1):151-6.
Biochem Biophys Res Commun. 1997 Feb 13;231(2):356-9.
Biochem Biophys Res Commun. 2002 Feb 1;290(4):1220-7.
Exp Mol Pathol. 2002 Jun;72(3):186-95.
Gene. 1991 Aug 15;104(2):177-85.
Genes Dev. 1992 Oct;6(10):1821-31.
Invest Ophthalmol Vis Sci. 1999 May;40(6):1071-80.
J Comp Neurol. 2001 Feb 19;430(4):485-500.
J Invest Dermatol. 1998 Dec;111(6):930-5.
J Neurosci. 1995 Mar;15(3 Pt 1):1971-83.
Perspect Dev Neurobiol 1994;2(1):111-16.

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Vis. Sci., 40, 1071-1080 (1999).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Tenascin-C secreted by transdifferentiated retinal pigment epithelial cells promotes choroidal neovascularization via integrin αV. Lab. Invest. (2016).(27668890)
Kobayashi Y, Yoshida S, Zhou Y, Nakama T, Ishikawa K, Kubo Y, Arima M, Nakao S, Hisatomi T, Ikeda Y, Matsuda A, Sonoda KH, Ishibashi T.