| RBRC No. | RBRC00107 |
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|---|---|---|---|---|---|---|---|---|---|
| Type | Targeted Mutation Congenic |
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| Species | Mus musculus | ||||||||
| Strain name | C3.Cg-Trp53<tm1Sia>/Rbrc | ||||||||
| Former Common name | C3H/HeN-TgH(p53) | ||||||||
| H-2 Haplotype | No Data | ||||||||
| ES cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] | ||||||||
| Donor strain | (C57BL/6 x CBA)F1 via TT2 ES cell line | ||||||||
| Background strain | C3H/HeNCrlCrlj | ||||||||
| Appearance | |||||||||
| 1 | Appearance | agouti | |||||||
| Genotype | No Data | ||||||||
| Strain development | Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences. | ||||||||
| Strain description | Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background). | ||||||||
| Colony maintenance | Heterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj] | ||||||||
| Health Report | No Data | ||||||||
| Gene Details | |||||||||
| Promoter | No Data | ||||||||
| 1 | Symbol | Trp53 | |||||||
| Symbol name | transformation-related protein 53 | ||||||||
| Chromosome | 11 | ||||||||
| Common name | p53 | ||||||||
| Symbol description | No Data | ||||||||
| Promoter | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||||||
| 2 | Symbol | neo | |||||||
| Symbol name | neomycin resistance gene (E. coli) | ||||||||
| Chromosome | 11 | ||||||||
| Common name | neo; neomycin; | ||||||||
| Symbol description | No Data | ||||||||
| References | Oncogene. 1993 Dec;8(12):3313-22. | ||||||||
| Research applications | Cancer Research, Cell Biology Research, Immunology and Inflammation Research, Mouse Models for Human Disease |
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| Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) | ||||||||
| Additional information | |||||||||
| 1 | Mouse of the Month Jun 2005 | ||||||||
| 2 | Genotyping protocol <PCR> | ||||||||
| Depositor | Niwa, Ohtsura (Radiation Biology Center, Kyoto University)  |
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| Strain Status / Availability (Expected delivery) |
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| Title | Journal (PMID) |
|---|---|
| Author | |
| Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells. | J Radiat Res (Tokyo).49(3): 231-40 (2008).(18285661) |
| Sugihara T, Murano H, Tanaka K, Oghiso Y. | |
| p53-Mediated Gene Activation in Mice at High Doses of Chronic Low-Dose-Rate gamma Radiation. | Radiat Res. (2010).(21189076) |
| Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K. | |
| Activation of Interferon-Stimulated Genes by {gamma}-Ray Irradiation Independently of the Ataxia Telangiectasia Mutated-p53 Pathway. | Mol Cancer Res.9(4): 476-84 (2011).(21357441) |
| Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K. | |
| p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation. | Radiat. Res.175(3): 328-35 (2011).(21388276) |
| Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K. |