RBRC00107, C3.Cg-Trp53<tm1Sia>/Rbrc

RBRC No. RBRC00107
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name C3.Cg-Trp53<tm1Sia>/Rbrc
Former Common name C3H/HeN-TgH(p53)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C3H/HeNCrlCrlj
1 Appearance agouti
Genotype No Data
Strain development Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain description Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance Heterozygote x Wild-type [or Crossing to C3H/HeNCrlCelj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Oncogene. 1993 Dec;8(12):3313-22.

Research applications Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 Mouse of the Month Jun 2005
2 Genotyping protocol <PCR>
Depositor Niwa, Ohtsura (Radiation Biology Center, Kyoto University) Niwa, Ohtsura
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Inverse dose-rate-effects on the expressions of extra-cellular matrix-related genes in low-dose-rate gamma-ray irradiated murine cells. J Radiat Res (Tokyo).49(3): 231-40 (2008).(18285661)
Sugihara T, Murano H, Tanaka K, Oghiso Y.
p53-Mediated Gene Activation in Mice at High Doses of Chronic Low-Dose-Rate gamma Radiation. Radiat Res. (2010).(21189076)
Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
Activation of Interferon-Stimulated Genes by {gamma}-Ray Irradiation Independently of the Ataxia Telangiectasia Mutated-p53 Pathway. Mol Cancer Res.9(4): 476-84 (2011).(21357441)
Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.
p53-Mediated gene activation in mice at high doses of chronic low-dose-rate γ radiation. Radiat. Res.175(3): 328-35 (2011).(21388276)
Sugihara T, Murano H, Nakamura M, Ichinohe K, Tanaka K.