RBRC00159, C57BL/6-p53

RBRC No. RBRC00159
Type Targeted Mutation and TransgeneCartagena 4wks
Species Mus musculus
Strain name C57BL/6-p53 tm1Sia-Tg(gpt Delta)
Former Common name C57BL/6-TgH(p53)-TgN(gpt delta)
H-2 Haplotype No Data
Donor strain Trp53: B6.CB-Trp53tm1Sia, gpt[Delta]: C57BL/6-Tg(gpt[Delta])
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a
Strain development The original p53 deficient mice had been introduced into C57BL/6 background for 13 generations by Dr. Niwa T at Hiroshima Univ (-1995). Then the mice were transfered to Dr. Kusakabe M. at RIKEN Life Science Center for further backcrossing to establish the B6 congenic strain. The gpt delta mice were crossed with C57BL/6-p53tm1 to establish a double mutant at RIKEN, and have been maintained by sister-brother mating for 8 generations.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
2 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol gpt Delta
Symbol name guanine phosphoribosyltransferase
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
6 Symbol red/gam
Symbol name lambda phage red/gam
Chromosome 17
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Environ. Mutagen Res., 22, 85-90 (2000).
Cancer Lett. 1999 Sep 1;143(2):241-4.
Cancer Res. 2001 May 15;61(10):3913-8.
Carcinogenesis. 2000 Nov;21(11):2049-56.
Environ Mol Mutagen. 1996;28(4):465-70.
Environ Mol Mutagen. 1999;34(1):1-8.
Environ Mol Mutagen. 1999;34(1):9-15.
Environ Mol Mutagen. 1999;34(2-3):72-9.
Oncogene. 1993 Dec;8(12):3313-22.
Phys Med 2001;17 Suppl 1:192-3.
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4156-61.

Research applications Apoptosis Research,
Cancer Research,
Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease,
Mouse Models for Human Disease,
Toxicology Research
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
High susceptibility of nullizygous p53 knockout mice to colorectal tumor induction by 1,2-dimethylhydrazine. J. Cancer Res. Clin. Oncol.129: 335-340 (2003).(12743811)
Sakai H, Tsukamoto T, Yamamoto M, Shirai N, Iidaka T, Hirata A, Yanai T, Masegi T, Donehower LA, Tatematsu M.
Low dose genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in gpt delta transgenic mice. Mutat. Res.541: 91-102 (2003).(14568298)
Ken-ichi MASUMURA, Mieko HORIGUCHI, Akiyoshi NISHIKAWA, Takashi UMEMURA, Keita KANKI, Yusuke KANKE, Takehiko NOHMI
Induction of colon tumors in C57BL/6J mice fed MeIQx, IQ, or PhIP followed by dextran sulfate sodium treatment. Toxicol. Sci.84: 243-248 (2005).(15635146)
Akiyoshi NISHIKAWA, Takayoshi IMAZAWA, Yuichi KUROIWA, Yasuki KITAMURA, Keita KANKI, Yuji ISHII, Takashi UMEMURA, Masao HIROSE