RBRC00169, B6.Cg-Tnc<tm1Sia>/Rbrc

RBRC No. RBRC00169
Type Targeted Mutation CongenicCartagena 12wks,♂
Species Mus musculus
Strain name B6.Cg-Tnc<tm1Sia>/Rbrc
Former Common name C57BL/6N-TgH(Tnc), B6-TN, TNKO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6NJcl
Appearance
1 Appearance black
Genotype a/a, B/B, C/C
Strain development Congenic strains were developed by Dr. M. Kusakabe, Cell Biology Laboratory, Life Science Tsukuba Research Center, RIKEN since 1992. A lacZ gene and neomycin resistant cassette was transfered into TT2 ES cells to replace the exon 2, just before the translation initiation codon of the Tnc gene. This strain was backcrossed to C57BL/6N.
Strain description Tenascin C knock-out mice. This strain is useful for studying the biological function of extracellular matrix tenascin C in vivo, especially in the field of tumor development, tumor metastasis, wound healing, tissue regeneration, angiogenesis, tissue interaction etc. The tenascin C deficient congenic strains were developed by successive backcrossing with A/J (RBRC00007), BALB/cA (RBRC00067), C57BL/6N (RBRC00169), C3H/HeN (RBRC00110) and GRS/A (RBRC00193).
Colony maintenance Homozygote x Homozygote
Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Tnc
Symbol name tenascin C
Chromosome 4
Common name Tnc; tenascin C, Hxb, Ten, TN-C, AI528729
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 4
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Am J Pathol. 1998 May;152(5):1237-45.
Biochem Biophys Res Commun. 1996 Apr 5;221(1):151-6.
Biochem Biophys Res Commun. 1997 Feb 13;231(2):356-9.
Biochem Biophys Res Commun. 2002 Feb 1;290(4):1220-7.
Exp Mol Pathol. 2002 Jun;72(3):186-95.
Gene. 1991 Aug 15;104(2):177-85.
Genes Dev. 1992 Oct;6(10):1821-31.
Invest Ophthalmol Vis Sci. 1999 May;40(6):1071-80.
J Comp Neurol. 2001 Feb 19;430(4):485-500.
J Invest Dermatol. 1998 Dec;111(6):930-5.
J Neurosci. 1995 Mar;15(3 Pt 1):1971-83.
Jpn J Cancer Res. 2002 Sep;93(9):968-75.
Perspect Dev Neurobiol 1994;2(1):111-16.

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Am. J. Pathol., 152, 1237-1245 (1998). J. Invest. Dermatol., 111, 930-935 (1998). Invest. Ophthalmol. Visual. Sci., 1071-1080 (1999).
Additional information
1 Genetic Background
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Invasion of melanoma in double knockout mice lacking tenascin-X and tenascin-C. Jpn. J. Cancer Res.93: 968-975 (2002).(12359049)
Matsumoto K, Takahashi K, Yoshiki A, Kusakabe M, Ariga H.
The dynamic expression of tenascin-C and tenascin-X during early heart development in the mouse. Differentiation71: 291-298 (2003).(12823230)
Kyoko IMANAKA-YOSHIDA, Kenn-ichi MATSUMOTO, Mari HARA, Teruyo SAKAKURA, Toshimichi YOSHIDA
Extracellular matrix protein Tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood. (2012).(22553313)
Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y.
Integrin alpha 9 on lymphatic endothelial cells regulates lymphocyte egress. Proc Natl Acad Sci U S A.111(8): 429647 (2014).(24516133)
Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T.