RBRC00235, B6.129X1-Chrng<tm1Mtak>/10Rbrc

RBRC No. RBRC00235
Type Targeted Mutation CongenicCartagena 8wks,♂
Species Mus musculus
Strain name B6.129X1-Chrng<tm1Mtak>/10Rbrc
Former Common name Achr, γ
H-2 Haplotype No Data
ES cell line RW4 [129X1/SvJ]
Donor strain 129X1/SvJ via RW4 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Dr. Masazumi Takahashi. This strain has been introduced into C57BL/6 genetic background by successive backcrossing for more than 10 generations in RIKEN Tsukuba Institute.
Strain description The acetylcholine receptors are known to have important roles in neuro-muscular functions. The gamma subunit are expressed in embryonic stages and the epsilon subunit are expressed postnatal stages.
The acetylcholine receptors gamma and epsilon subunits have been disrupted by homologous recombination to study the functions of these genes and switching mechanism of gamma to epsilon subunits. The gamma subunit deficient homozygotes died after two days post delivery, suffering from inability of motor function of the limbs and drinking milk. The analysis on the neuro-muscular junctions showed the structural and electrophysiological abnormalities.
Colony maintenance Backcrossing to C57BL/6. Or sister-brother mating of heterozygotes. Breeding performance is good.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Chrng
Symbol name cholinergic receptor, nicotinic, gamma polypeptide
Chromosome 1
Common name Achr-3, Acrg
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Development. 1997 Dec;124(24):5075-86.
EMBO Rep. 2002 Jul;3(7):674-81.
FEBS Lett. 1987 Oct 19;223(1):104-12.
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13286-91.
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13413-8.

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Takahashi, Masazumi (Centre National de Genotypage) Takahashi, Masazumi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Essential roles of the acetylcholine receptor gamma-subunit in neuromuscular synaptic patterning. Development.135(11): 1957-67 (2008).(18434415)
Liu Y, Padgett D, Takahashi M, Li H, Sayeed A, Teichert RW, Olivera BM, McArdle JJ, Green WN, Lin W.