RBRC00254, C57BL/6-TgN(a-CaMKII-nlCre)/20

RBRC No. RBRC00254
Type Transgene CongenicCartagena 6wks,♀
Species Mus musculus
Strain name C57BL/6-TgN(a-CaMKII-nlCre)/20
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype No Data
Strain development Developed by Futatsugi A. The Cre-Strain series were transfered to Experimental Animal Division, RIKEN Tsukuba Institute in 1999.
Strain description The Cre-recombinase activity was detected specifically in the nuclear fraction of the cerebrum. The expression level of the cre in each line of Tg mice was following order; Line 10, 26> 22, 17N > 17A, 11, 20. The expression of the cre was detected by RT-PCR (including semi-quantitative
one), Western blotting and the Cre-recombinase activity assay. There are some variation in the amount of expression in the cerebrum and leaky expression (less than 1/10 to 1/100 of that in the cerebrum at maximum) in the cerebellum among the different lines.
Colony maintenance Maintained by sister-brother mating. Breed well, Hemizygote x Wild-type, or hemizygote hemizygote.
Health Report
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Camk2a promoter
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall prior contact with the DEPOSITOR, discussn use of it and obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and NOT for commercial use. The RECIPIENT can use the BIOLOGICAL RESOURCE in a collaboration basis with the DEPOSITOR, except for some cases where only ACKNOLEGEMENT is requested by citing the DEPOSITOR. BEFORE the publication on the BIOLOGICAL RESOURCE by the DEPOSITOR, the RECIPIENT agrees to use the BIOLOGICAL RESOURCE in collaboration with the DEPOSITOR and add Drs. Akira Futatsugi and Katsuhiko Mikoshiba as a co-authors. The RECIPIENT agrees to cite publications requested by the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Mikoshiba, Katsuhiko (RIKEN Center for Brain Science) Mikoshiba, Katsuhiko
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Generation of a conditional knockout allele for mammalian Spen protein Mint/SHARP. Genesis.45(5): 300-6 (2007).(17457934)
Yabe D, Fukuda H, Aoki M, Yamada S, Takebayashi S, Shinkura R, Yamamoto N, Honjo T.
Prox1 postmitotically defines dentate gyrus cells by specifying granule cell identity over CA3 pyramidal cell fate in the hippocampus. Development.139(16): 3051-62 (2012).(22791897)
Iwano T, Masuda A, Kiyonari H, Enomoto H, Matsuzaki F.