RBRC00398, GelA KO

RBRC No. RBRC00398
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name GelA KO
Former Common name GelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.
Strain description This strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Colony maintenance Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Health Report
Gene Details
Promoter No Data
1 Symbol Mmp2
Symbol name Matrix metalloproteinase 2
Chromosome 8
Common name 72 KDa gelatinase, 72 KDa type III collagenase, Clg4a, GelA, gelatinase A, MMP-2
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References J Biol Chem. 1997 Sep 5;272(36):22389-92.

Research applications Cardiovascular Research,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 272, 22389-22392 (1997).
Additional information
1 Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction. Am. J. Physiol. Heart. Circ. Physiol.285: H1229-1235 (2003).(12775562)
Shunji HAYASHIDANI, Hiroyuki TSUTSUI, Masaki IKEUCHI, Tetsuya SHIOMI, Hidenori MATSUSAKA, Toru KUBOTA, Kyoko IMANAKA-YOSHIDA, Takeshi ITOH, Akira TAKESHITA
Selective Disruption of Matrix Metalloproteinase-2 Gene Exacerbates Myocardial Inflammation and Dysfunction in Mice with Cytokine-Induced Cardiomyopathy. Am. J. Physiol. Heart. Circ. Physiol.289: H1858-1864 (2005).(15937097)
Hidenori MATSUSAKA, Masaki IKEUCHI, Shouji MATSUSHIMA, Tomomi IDE, Toru KUBOTA, Arthur M FELDMAN, Akira TAKESHITA, Kenji SUNAGAWA, Hiroyuki TSUTSUI
Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis. J Exp Med.203(4): 1007-19 (2006).(16585265)
Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts. Am J Pathol.173(3): 781-91 (2008).(18669618)
Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2. Kidney Int.78(1): 69-78 (2010).(20375993)
Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice. Nephrol Dial Transplant. (2012).(23028104)
Takamiya Y, Fukami K, Yamagishi SI, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway. Redox Biol.4C: 169-179 (2014).(25574848)
Cinq-Frais C, Coatrieux C, Savary A, D’Angelo R, Bernis C, Salvayre R, N gre-Salvayre A, Aug N.
Matrix metalloproteinase 14 is required for fibrous tissue expansion. Elife (2015).(26390284)
Taylor SH, Yeung CC, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.