RBRC00408, B6.129P2-Gfap<tm1Ito>/ItoRbrc

RBRC No. RBRC00408
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.129P2-Gfap<tm1Ito>/ItoRbrc
Former Common name Gfap KO, GFAP-KO-clone 27
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
1 Appearance black
Genotype No Data
Strain development Developed by Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1995. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed to C57BL/6J.
Strain description Gfap knockout mice. Immediately downstream of the endogenous start codon of Gfap was replaced with a cassette, containing a promoterless lacZ and pgk-neo. Gfap, glial fibrillary acidic protein is an intermediate filament protein specifically expressed in astrocytes. Homozygous mutant mice develop normally and not show any detectable abnormalities in the anatomy of the brain. But, exhibit a impairment of long-term depression (LTD) and PF-Purkinje cell synapses and eyeblink, suggested that the Gfap is required for communications between Bergmann glia and Purkinje cells.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Gfap
Symbol name glial fibrillary acidic protein
Chromosome 11
Common name No Data
Symbol description No Data
Promoter endogenous start codon
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Neuron. 1995 Jan;14(1):29-41.

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Gomi et al., Neuron, 14, 29-41 (1995).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP. Genes Dev.22(4): 489-98 (2008).(18281462)
Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.