RBRC00438, SEK1 Knockout mice

RBRC No. RBRC00438
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name SEK1 Knockout mice
Former Common name SEK1 Knockout mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Hiroshi Nishina at Ontario Cancer Institute, University of Toronto in 1997. A neomycin selection cassette was inserted into the exon 2 of the SEK1 gene. The SEK1 deficient mice were backcrossed to C57BL/6 over 13 times.
Strain description B6.129P-Map2k4<tm1Pngr>. The stress signaling kinase SEK1/MKK4 is a direct activator of stress-activated protein kinase, or Jun N-terminal kinases (SAPKs/JNKs) in response to environmental stress or mitogenic factors. Homozygous mutant mice exhibit severe anemia and die between embryonic day 10.5 and 12.5. This mutant strain is useful for the study of SEK1 gene function.
Colony maintenance Backross to C57BL/6 (Heterozygote x C57BL/6J)
Homozygous mutant mice die between embryonic day 10.5 and 12.5.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Map2k4
Symbol name mitogen activated protein kinase kinase 4
Chromosome 11
Common name MEK4, MKK4, Sek1, JNKK1, Serk1, PRKMK4
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Dev Biol. 2002 Oct 15;250(2):332-47.
Development. 1999 Feb;126(3):505-16.
Nature. 1997 Jan 23;385(6614):350-3.

SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis.
SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis.
SEK1/MKK4-mediated SAPK/JNK signaling participates in embryonic hepatoblast proliferation via a pathway different from NF-kappaB-induced anti-apoptosis.
Research applications Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Nishina, Hiroshi (Medical Research Institute, Tokyo Medical and Dental University) Nishina, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Hepatoblasts comprise a niche for fetal liver erythropoiesis through cytokine production. Biochem Biophys Res Commun. (2011).(21664343)
Sugiyama D, Kulkeaw K, Mizuochi C, Horio Y, Okayama S.
TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts. Mech Dev. (2012).(23041440)
Sugiyama D, Kulkeaw K, Mizuochi C.