RBRC00445, C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb

RBRC No. RBRC00445
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common name C57BL/6-TgN(acro/act-EGFP)OsbC3-N01-FJ002
H-2 Haplotype No Data
Background strain C57BL/6NCrSlc
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The CAG and acr-EGFP plasmids were co-injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6NCr background.
Strain description Transgenic mice expressing GFP ubiquitously from CAG-EGFP and in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG-EGFP, in which EGFP is connected to the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG).
Colony maintenance Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc].
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Curr Top Dev Biol. 1999;44:1-20.
Dev Biol. 2001 Sep 1;237(1):222-31.
FEBS Lett. 1997 May 5;407(3):313-9.
FEBS Lett. 1999 Apr 23;449(2-3):277-83.

Research applications Fluorescent Proteins/lacZ System,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 237, 222-231 (2001).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Mol Cell Biol.27(19): 6794-805 (2007).(17664285)
Lin YN, Roy A, Yan W, Burns KH, Matzuk MM.
Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proc Natl Acad Sci U S A. (2012).(22984182)
Sato T, Yokonishi T, Komeya M, Katagiri K, Kubota Y, Matoba S, Ogonuki N, Ogura A, Yoshida S, Ogawa T.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem. Biophys. Res. Commun. (2018).(29705697)
Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.