RBRC00752, B6.129P2-Tnfrsf5<tm1Kik>/Rbrc

RBRC No. RBRC00752
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Tnfrsf5<tm1Kik>/Rbrc
Former Common name CD40 Knockout mice
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14.1 ES cell line
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Hitoshi Kikutani, Institute for Molecular and Cellular Biology, Osaka University in 1994.
Strain description CD40 knockout mice. Exon 3 of the CD40 was replaced with a neo cassette. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation. Homozygous mutant mice for CD40 targeted mutation exhibit impaired immunoglobulin class switching, and the germinal center formation was defective. Homozygous mice are viable and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Tnfrsf5
Symbol name tumor necrosis factor receptor superfamily, member 5
Chromosome 2
Common name Bp50, Cd40, IGM, p50
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References Immunity. 1994 Jun;1(3):167-78.

The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Immunity, 1, 167-178 (1994).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kikutani, Hitoshi (Osaka university) Kikutani, Hitoshi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months