RBRC00771, C57BL/6J-Tg(GOFGFP)11Imeg

RBRC No. RBRC00771
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(GOFGFP)11Imeg
Former Common name Oct-GFP#11, TgN(GOFGFP)11Imeg
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype No Data
Strain development Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 2001. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain description Oct-4/EGFP transgenic mice carry EGFP (enhanced green fluorescence protein) cDNA under control of an Oct4 18-kb genomic fragment containing the minimal promoter and proximal and distal enhanced. (Dev Biol 2003). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenance Carrier x Carrier [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter Oct 3/4 promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Dev Biol. 2003 Jun 1;258(1):209-25.

Identification and characterization of stem cells in prepubertal spermatogenesis in mice small star, filled.
Identification and characterization of stem cells in prepubertal spermatogenesis in mice small star, filled.
Research applications Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003).
The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Abe, Kuniya (RIKEN, BioResource Center) Abe, Kuniya
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Mature oocytes derived from purified mouse fetal germ cells. Hum Reprod.23(1): 54-61 (2008).(18000170)
Qing T, Liu H, Wei W, Ye X, Shen W, Zhang D, Song Z, Yang W, Ding M, Deng H.
Novel variants of Oct-3/4 gene expressed in mouse somatic cells. J Biol Chem.283(45): 30997-1004 (2008).(18765667)
Mizuno N, Kosaka M.
Pluripotin combined with leukemia inhibitory factor greatly promotes the derivation of embryonic stem cell lines from refractory strains. Stem Cells.27(2): 383-9 (2009).(19056907)
Yang W, Wei W, Shi C, Zhu J, Ying W, Shen Y, Ye X, Fang L, Duo S, Che J, Shen H, Ding S, Deng H.
Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system. Dev Biol.346(1): 90-101 (2010).(20659443)
Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T.
Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. Cell Res.21(1): 196-204 (2011).(20956998)
Li Y, Zhang Q, Yin X, Yang W, Du Y, Hou P, Ge J, Liu C, Zhang W, Zhang X, Wu Y, Li H, Liu K, Wu C, Song Z, Zhao Y, Shi Y, Deng H.