RBRC00782, B6.129P2-Gipr<tm1Yse>/Rbrc

RBRC No. RBRC00782
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Gipr<tm1Yse>/Rbrc
Former Common name GIPR Knockout mice
H-2 Haplotype No Data
Donor strain 129P2/Ola via CGR8 ES cell line
Background strain C57BL/6NCrSlc
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Yutaka Seino, Kyoto University in 1999. A neomycin cassette was inserted to replace exons 4 and 5 of GIPR gene. The mutant mice were backcrossed to C57BL/6.
Strain description B6.129P2-Gipr<tm1Yse>. Gastric inhibitory polypeptide (GIP) is a gastrointestinal peptide hormone synthesized by K cells in the duodenum and small intestine, GIP plays a role in gastric secretory inhibition, gastric mobility, and insulin release by binding to its specific receptor, the GIP receptor (GIPR). Homozygous mutant mice for Gipr<tm1Yse> targeted mutation are fertile and viable, but exhibit hyperglycemia after high-fat diet ingestion. This strain is useful for elucidating of the signaling mechanism of GIP from pancreatic beta cell to intestine.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
Homozygous mice are fertile and viable.
Health Report
Gene Details
Promoter No Data
1 Symbol Gipr
Symbol name gastric inhibitory polypeptide receptor
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14843-7.

Glucose intolerance caused by a defect in the entero-insular axis: a study in gastric inhibitory polypeptide receptor knockout mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Miyawaki K, et al. Proc Natl Acad Sci USA, 96, 14843-14847 (1999).
Additional information
1 Genetic Background
2 Genotyping protocol <PCR>
Depositor Seino, Yutaka (Kyoto University) Seino, Yutaka
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin. Diabetologia (2016).(27053237)
Iida A, Seino Y, Fukami A, Maekawa R, Yabe D, Shimizu S, Kinoshita K, Takagi Y, Izumoto T, Ogata H, Ishikawa K, Ozaki N, Tsunekawa S, Hamada Y, Oiso Y, Arima H, Hayashi Y.