RBRC00807, B6.129S2-Ercc5<tm1Shm>/ShmRbrc

RBRC No. RBRC00807
Type Targeted Mutation CongenicCartagena 8wks,♂
Species Mus musculus
Strain name B6.129S2-Ercc5<tm1Shm>/ShmRbrc
Former Common name Xpg/2 Knockout mice
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain C57BL/6NCrSlc
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. T. Shiomi, National Institute of Radiological sciences in 1999. A neomycin selected cassette was transfered into D3 ES cels to replace the exon 3 of Ercc5 gene. C57BL/6Cr background.
Strain description B6.129S2-Ercc5<tm1Shm>. Xeroderma pigmentosum group G (XPG) gene is a structure-specific DNA endonuclease which plays a role in nucleotide exision repair and transcription-coupled repair of oxidative DNA damage. Xeroderma pigmenrosum (XP) is a autosomal recessive disorder characterized by hypersensitivity to sunlight, abnomal pigmentation, and predisposition to skin cancers, and caused by defects in an early step of the nucleotide exicision repair pathway. Xpg2 deficient mutant mice exhibit severely postnatal growth failure and underwent premature death. This strain is useful as a animal model for Xeroderma Pigmentosum.
Colony maintenance Sibling Mating (Heterozygote x Wild-type)
Homozygous mutant mice display severely postnatal growth failure and underwent premature death.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ercc5
Symbol name excision repair cross-complementing rodent repair deficiency,complementation group 5
Chromosome 1
Common name Xpg
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 1999 Mar;19(3):2366-72.

Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G gene.
Research applications Cell Biology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Shiomi, Tadahiro (National Institute of Radiological Sciences) Shiomi, Tadahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months