RBRC00808, B6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc

RBRC No. RBRC00808
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc
Former Common name Emx1-Cre
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black (a/a B/B C/C)
Genotype No Data
Strain development Developed by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J.
Strain description Emx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk-neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Emx1
Symbol name empty spiracles homolog 1 (Drosophila)
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 poly A
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter
5 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Nature. 2000 Aug 17;406(6797):726-31.

Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 406, 726-731 (2000).
Additional information
1 Lab HP
2 Mouse of the Month June 2006
3 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Hes1 and Hes5 control the progenitor pool, intermediate lobe specification, and posterior lobe formation in the pituitary development. Mol Endocrinol.21(6): 1458-66 (2007).(17426285)
Kita A, Imayoshi I, Hojo M, Kitagawa M, Kokubu H, Ohsawa R, Ohtsuka T, Kageyama R, Hashimoto N.
Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline. Development.135(15): 2531-41 (2008).(18579678)
Imayoshi I, Shimogori T, Ohtsuka T, Kageyama R.
Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis. PLoS One.3(12): e3859 (2008).(19050759)
Kinameri E, Inoue T, Aruga J, Imayoshi I, Kageyama R, Shimogori T, Moore AW.