RBRC00815, MSM.Cg-Trp53<tm1Sia>/JpkRbrc

RBRC No. RBRC00815
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus molossinus
Strain name MSM.Cg-Trp53<tm1Sia>/JpkRbrc
Former Common name MSM-p53 KO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain B6.Cg-Trp53tm1Sia (と思われる)
Background strain MSM/Ms
1 Appearance white-bellied agouti
Genotype A/A B/B C/C
Strain development Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain description Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance Heterozygote x Wild-type [or Crossing to MSM/Ms]
Health Report
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Jpn J Cancer Res. 1996 Jul;87(7):696-701.
Oncogene. 1993 Dec;8(12):3313-22.

P53 gene deficiency does not enhance instability of mouse minisatellites in somatic cells of normal tissues.
P53 gene deficiency does not enhance instability of mouse minisatellites in somatic cells of normal tissues.
Research applications Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 Mouse of the Month Jun 2005
2 Genotyping protocol <PCR>
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months