RBRC00821, C57BL/6J-Tg(deGFP)18Imeg

RBRC No. RBRC00821
Type TransgeneCartagena 6wks,♀
Species Mus musculus
Strain name C57BL/6J-Tg(deGFP)18Imeg
Former Common name Oct-delta PE-GFP #18, TgN(deGFP)18Imeg
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype No Data
Strain development Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822).
Strain description Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Oct 3/4 promoter
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Dev Biol. 2003 Jun 1;258(1):209-25.

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003).
The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
Additional information
1 Mouse of the Month Sep 2005
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
3 Genotyping protocol <PCR>
Depositor Abe, Kuniya (RIKEN, BioResource Center) Abe, Kuniya
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Birth of mice produced by germ cell nuclear transfer. Genesis41: 81-86 (2005).(15712265)
Hiromi MIKI, Kimiko INOUE, Takashi KOHDA, Arata HONDA, Narumi OGONUKI, Misako YUZURIHA, Nathan MISE, Yasuhisa MATSUI, Tadashi BABA, Kuniya ABE, Fumitoshi ISHINO, Atsuo OGURA
Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells.13(8): 863-77 (2008).(18782224)
Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice. PLoS Genet.5(12): e1000756 (2009).(19997484)
Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells. Stem Cells Dev. (2011).(21861689)
Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K.
Molecular Identification of t(w5): Vps52 Promotes Pluripotential Cell Differentiation through Cell-Cell Interactions. Cell Rep. (2012).(23142660)
Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
Large, Male Germ Cell-Specific Hypomethylated DNA Domains With Unique Genomic and Epigenomic Features on the Mouse X Chromosome. DNA Res. (2013).(23861320)
Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.