RBRC00850, B6.129P2-Itgal

RBRC No. RBRC00850
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129P2-Itgaltm1Mak
Former Common name C57BL/6-[KO]LFA-1; B6.129P2-Itgb2tm1Oci
H-2 Haplotype No Data
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype No Data
Strain development From Tak. W. Mak of ontario Cancer Institute, N6
Strain description Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Itgal
Symbol name integrin alpha L
Chromosome 7
Common name CD11a, Integrin alpha L
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1996 Apr 1;183(4):1415-26.

LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells. Nat Commun8(1): 1592 (2017).(29150602)
Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K.