RBRC00858, B6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc

RBRC No. RBRC00858
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc
Former Common name IRF-3 KO/B6; IRF-3 Knockout mice, C57BL/6J
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEv via CCE ES cell line
Background strain C57BL/6JJcl
1 Appearance black
Genotype No Data
Strain development Developed by Tadatsugu Taniguchi, Graduate School of Medicine and Faculty of Medicine, University of Tokyo. C57BL/6 semicongenic strain.
Strain description The Irf3 deficient mice were created by replacing a 2.2 kb region of Irf3 containing a putative transcription initiation site and the coding N-terminal region of the DNA binding domain. The analysis of MEFs demonstrated loss of Irf3 protein. Homozygous null mice are more susceptible to viral infection such as encephalomyocarditis virus and exhibit lower serum interferon levels in response to viral infection, indicating incapability of the mutant to respond against viral infection.
Colony maintenance Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Bcl2l12
Symbol name BCL2-like 12 (proline rich)
Chromosome 7
Common name Bcl2-L12, Bcl-L12
Symbol description No Data
2 Symbol Irf3
Symbol name interferon regulatory factor 3
Chromosome 7
Common name C920001K05Rik, IRF-3
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Immunity. 2000 Oct;13(4):539-48.

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
Additional information
1 Taniguchi Lab HP
2 Mouse of the Month May 2005
Genetic Background
3 Genotyping protocol <PCR>
Depositor Taniguchi, Tadatsugu (The University of Tokyo, Graduate School of Medicine) Taniguchi, Tadatsugu
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation. Blood.107(3): 1078-84 (2006).(16219795)
Goriely S, Molle C, Nguyen M, Albarani V, Haddou NO, Lin R, De Wit D, Flamand V, Willems F, Goldman M.
Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice. Virology.359(1): 82-91 (2007).(17027894)
Kiyotani K, Sakaguchi T, Kato A, Nagai Y, Yoshida T.
IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3. J Immunol.178(12): 7607-15 (2007).(17548596)
Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S.
Double-stranded RNA induces pancreatic beta-cell apoptosis by activation of the toll-like receptor 3 and interferon regulatory factor 3 pathways. Diabetes.57(5): 1236-45 (2008).(18223009)
Dogusan Z, Garc a M, Flamez D, Alexopoulou L, Goldman M, Gysemans C, Mathieu C, Libert C, Eizirik DL, Rasschaert J.
Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system. Proc Jpn Acad Ser B Phys Biol Sci.84(10): 439-51 (2008).(19075516)
Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y.
Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner. J Virol.83(15): 7629-40 (2009).(19474102)
Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y.
Systemic lipopolysaccharide protects the brain from ischemic injury by reprogramming the response of the brain to stroke: a critical role for IRF3. J Neurosci.29(31): 9839-49 (2009).(19657036)
Marsh B, Stevens SL, Packard AE, Gopalan B, Hunter B, Leung PY, Harrington CA, Stenzel-Poore MP.
Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process. J Immunol.184(4): 1784-92 (2010).(20083668)
Molle C, Goldman M, Goriely S.
Stimulator of IFN gene is critical for induction of IFN-beta during Chlamydia muridarum infection. J Immunol.184(5): 2551-60 (2010).(20107183)
Prantner D, Darville T, Nagarajan UM.
Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus. J Virol.84(18): 9452-62 (2010).(20592086)
Zhou S, Cerny AM, Zacharia A, Fitzgerald KA, Kurt-Jones EA, Finberg RW.
Interferon response factor 3 is essential for house dust mite-induced airway allergy. J Allergy Clin Immunol.126(4): 836-844.e13 (2010).(20673978)
Marichal T, Bedoret D, Mesnil C, Pichavant M, Goriely S, Trottein F, Cataldo D, Goldman M, Lekeux P, Bureau F, Desmet CJ.
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways. Mol Immunol.48(1-3): 119-27 (2010).(20947170)
Warz e B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ.
The role of TBK1 and IKK in the expression and activation of Pellino 1. Biochem J.434(3): 537-48 (2011).(21204785)
Smith H, Liu XY, Dai L, Goh ET, Chan AT, Xi J, Seh CC, Qureshi IA, Lescar J, Ruedl C, Gourlay R, Morton S, Hough J, McIver EG, Cohen P, Cheung PC.
A conventional protein kinase C inhibitor targeting IRF-3-dependent genes differentially regulates IL-12 family members. Mol Immunol. (2011).(21550664)
Johnson J, Molle C, Aksoy E, Goldman M, Goriely S, Willems F.
Multiple preconditioning paradigms converge on interferon regulatory factor-dependent signaling to promote tolerance to ischemic brain injury. J Neurosci.31(23): 8456-63 (2011).(21653850)
Stevens SL, Leung PY, Vartanian KB, Gopalan B, Yang T, Simon RP, Stenzel-Poore MP.
IRF3 protects from uterine horn pathology during Chlamydia muridarum genital infection in mice. Infect Immun. (2011).(21788382)
Prantner D, Sikes JD, Hennings L, Savenka AV, Basnakian AG, Nagarajan UM.
Differential impact of interferon regulatory factor 7 in the initiation of the type I interferon response in the LCMV infected CNS versus the periphery. J Virol. (2012).(22514347)
Christensen JE, Fenger C, Issazadeh-Navikas S, Krug A, Liljestr m P, Goriely S, Paludan SR, Finsen B, Christensen JP, Thomsen AR.
IRF3 deficiency leads to IL-17-mediated liver ischemia-reperfusion injury. Hepatology. (2012).(22911673)
Loi P, Yuan Q, Torres D, Delbauve S, Laute MA, Lalmand MC, P tein M, Goriely S, Goldman M, Flamand V.
Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. Proc Natl Acad Sci U S A.110(34): E3189-97 (2013).(23918362)
Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S.
Interferon regulatory factor 3 protects against adverse neo-intima formation. Cardiovasc Res. (2014).(24596398)
Zhang SM, Zhu LH, Li ZZ, Wang PX, Chen HZ, Guan HJ, Jiang DS, Chen K, Zhang XF, Tian S, Yang D, Zhang XD, Li H.
IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide. J. Immunol.198(9): 3637-3649 (2017).(28363903)
Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N.