RBRC00886, B6;B6C3-Tg(Acro3-EGFP)01Osb

RBRC No. RBRC00886
Type TransgeneCartagena 6wks,♂
Species Mus musculus
Strain name B6;B6C3-Tg(Acro3-EGFP)01Osb
Former Common name B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01
H-2 Haplotype No Data
Background strain B6C3F1[C57BL/6N and C3H/HeN]
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. Acr3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mice were crossed to C57BL/6.
Strain description Transgenic mice expressing GFP in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter.
Colony maintenance Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References FEBS Lett. 1999 Apr 23;449(2-3):277-83.

Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
FEBS Lett., 449, 277-283 (1999).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
In vitro murine spermatogenesis in an organ culture system. Biol Reprod.83(2): 261-7 (2010).(20393168)
Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T.
In vitro production of functional sperm in cultured neonatal mouse testes. Nature.471(7339): 504-7 (2011).(21430778)
Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T.
In vitro production of fertile sperm from murine spermatogonial stem cell lines. Nat Commun.2: 472 (2011).(21915114)
Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T.
Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device. Sci. Rep. (2016).(26892171)
Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro. Sci Rep7(1): 15459 (2017).(29133858)
Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis. Radiat. Res. (2018).(29595376)
Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem. Biophys. Res. Commun. (2018).(29705697)
Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.