RBRC00891, B6.129P2-Spen<tm1Hon>/HonRbrc

RBRC No. RBRC00891
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Spen<tm1Hon>/HonRbrc
Former Common name MINT-KO (C57BL/6 background)
H-2 Haplotype No Data
Donor strain 129P2/OldHsd via E14 ES cell line
Background strain C57BL/6NCrSlc
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Tasuku Honjo, Kyoto University in 2003. A pgk-neo was transfered into E14 ES cells to replace nucleotide 4891 of the coding region of Mint. The Mint deficient mice were backcrossed to C57BL/6 for 16 times.
Strain description B6.129P2-Mint-pending<tm1Hon>/HonRbrc. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Homozygous mutant mice are embryonic lethal due to developmental abnormality of heart, spleen and liver. This strain is useful for elucidating of Notch/RBP-J signaling pathway mechanism in mammals.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
The reproductive performance is good. Homozygous mutant mice are embryonic lethal.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Spen
Symbol name Msx2-interacting nuclear target protein (MINT)
Chromosome 4
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Immunity. 2003 Feb;18(2):301-12.

Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway.
Research applications Developmental Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Immunity 18, 301-312 (2003).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months