Strain Information | |
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Image | |
BRC No. | RBRC00891 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Spen<tm1Hon>/HonRbrc |
Former Common name | MINT-KO (C57BL/6 background) |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Dr. Tasuku Honjo, Kyoto University in 2003. A pgk-neo was transfered into E14 ES cells to replace nucleotide 4891 of the coding region of Mint. The Mint deficient mice were backcrossed to C57BL/6 for 16 times. |
Strain description | B6.129P2-Mint-pending<tm1Hon>/HonRbrc. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Homozygous mutant mice are embryonic lethal due to developmental abnormality of heart, spleen and liver. This strain is useful for elucidating of Notch/RBP-J signaling pathway mechanism in mammals. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)The reproductive performance is good. Homozygous mutant mice are embryonic lethal. |
References | Immunity, 18, 301-312 (2003). 12594956 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse MINT genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo |
Research application | Developmental Biology Research Immunology and Inflammation Research |
Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Immunity 18, 301-312 (2003). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
Depositor | Tasuku Honjo (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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