RBRC00903, C.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)

RBRC No. RBRC00903
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name C.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)
Former Common name PD-1-KO-N10 (BALB/c)
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain BALB/c
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Tasuku Honjo, Kyoto University
Strain description The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygous mutant mice are fertile and viable.
Health Report
Gene Details
Promoter No Data
1 Symbol Pdcd1
Symbol name programmed cell death 1
Chromosome 1
Common name Ly101, PD-1, Pdc1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.
Science. 2001 Jan 12;291(5502):319-22.

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Mar 2005
2 Honjo Lab HP
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months