RBRC00980, B6C3F1-Tg(CAG-EGFP)CX-FM038Osb

RBRC No. RBRC00980
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)CX-FM038Osb
Former Common name 152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038
H-2 Haplotype No Data
Background strain Slc:B6C3F1
Appearance
1 Appearance black or agouti
Genotype a/a B/B C/C or A/? B/B C/C
Strain development Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.
Strain description The CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.
Colony maintenance Sibling Mating
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome X
Common name No Data
Symbol description No Data
References Curr Biol. 2006 Jan 24;16(2):166-72.
FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.

Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics, 80, 564-574 (2002). Curr Biol., 16(2):166-172 (2006).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos. Nucleic Acids Res.38(11): 3672-81 (2010).(20185572)
Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M.
DA-Raf-dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation. Proc Natl Acad Sci U S A. (2014).(24843139)
Watanabe-Takano H, Takano K, Sakamoto A, Matsumoto K, Tokuhisa T, Endo T, Hatano M.