RBRC00984, ICR;129P2-Nfe2l2<tm1Mym>/MymRbrc

RBRC No. RBRC00984
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR;129P2-Nfe2l2<tm1Mym>/MymRbrc
Former Common name Nrf2 knock out mouse (ICR background)
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain Jcl:ICR
1 Appearance No Data
Genotype No Data
Strain development Developed by Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 1996. The targeting vector containing lacZ-neo cassette was transferred into E14 ES cells to replace the 1.2 kb segment containing the rest of the exon 5 coding sequence of the Nrf2 gene.
Strain description Nrf2 (Nfe2l2) Knockout mice. This strain is a useful model for the in vivo analysis of chemical carcinogenesis and resistance to anti-cancer drugs. A phenolic antioxidant significantly induced the expression of phase II enzymes such as glutathione S-transferase and NAD(P)H: quinone oxidoreductase in normal mice. However, in the homozygous Nrf2 deficient mice the induction of the phase II enzymes by a phenolic antioxidant was largely eliminated in the liver and intestine. Since Nrf2 is constantly degraded by proteasome under normal condition, it is hardly to detect Nrf2 protein in the absence of stimuli. Electrophilic chemicals, such as DEM (diethyl maleate) and tBHQ (tert-buthyl hydroquinone), are generally used to stabilize Nrf2. Or proteasomal inhibitors, such as MG132, are also effective increasing the protein amount of Nrf2. C57BL/6 background (RBRC01390), BALB/c background (RBRC02190), A/J background (RBRC02414), ICR mixed background (RBRC00984). Homozygous mutant mice are viable and fertile, but show lower reproductive performance.
Colony maintenance Homozygote x Heterozygote [or Crossing to Jcl:ICR]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nfe2l2
Symbol name nuclear factor, erythroid derived 2, like 2
Chromosome 2
Common name Nrf2
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 2
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 2
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References Biochem Biophys Res Commun. 1997 Jul 18;236(2):313-22.

An Nrf2/small Maf heterodimer mediates the induction of phase II detoxifying enzyme genes through antioxidant response elements.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 236, 313-322 (1997).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The depositor should be a co-author in the articles when the users publish their data using these mice for 2 year after provided.
Any patent rights and other intellectual properties are retained by the depositor. Users should contact the depositor in the case of application for any patents with the results from these mice.
Additional information
1 Mouse of the Month Oct 2005
Mouse of the Month Apr 2012
2 Lab HP (Japanese)
3 Genotyping protocol <PCR>
Depositor Yamamoto, Masayuki (University of tsukuba) Yamamoto, Masayuki
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Dietary sulforaphane, a histone deacetylase inhibitor for cancer prevention. J Nutr.139(12): 178756 (2009).(19812222)
Ho E, Clarke JD, Dashwood RH.
The transcriptome of Nrf2-/- mice provides evidence for impaired cell cycle progression in the development of cigarette smoke-induced emphysematous changes. Toxicol Sci.115(1): 238-52 (2010).(20133372)
Gebel S, Diehl S, Pype J, Friedrichs B, Weiler H, Sch ller J, Xu H, Taguchi K, Yamamoto M, M ller T.
Metabolism and Tissue Distribution of Sulforaphane in Nrf2 Knockout and Wild-Type Mice. Pharm Res. (2011).(21681606)
Clarke JD, Hsu A, Williams DE, Dashwood RH, Stevens JF, Yamamoto M, Ho E.
Developmental role of nuclear factor E2-related factor 2 in mitigating methamphetamine fetal toxicity and postnatal neurodevelopmental deficits. Free Radic Biol Med.65C: 620-631 (2013).(23932974)
Ramkissoon A, Wells PG.
Methamphetamine Oxidative Stress, Neurotoxicity, And Functional Deficits Are Modulated By Nuclear Factor-E2-Related Factor 2. Free Radic. Biol. Med. (2015).(26427884)
Ramkissoon A, Wells PG.