| RBRC No. | RBRC00986 |
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| Type | Transgene |
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| Species | Mus musculus | |||||||||||||||||||||||||||||||
| Strain name | B6C3F1-Tg(CAG-EGFP)CX-FM50Osb | |||||||||||||||||||||||||||||||
| Former Common name | B6C3F1B6C3F1 TgN(act-EGFP)OsbCX-FM050 | |||||||||||||||||||||||||||||||
| H-2 Haplotype | No Data | |||||||||||||||||||||||||||||||
| Background strain | No Data | |||||||||||||||||||||||||||||||
| Appearance | ||||||||||||||||||||||||||||||||
| 1 | Appearance | black | ||||||||||||||||||||||||||||||
| Genotype | a/a B/B C/C | |||||||||||||||||||||||||||||||
| Strain development | Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs. | |||||||||||||||||||||||||||||||
| Strain description | Both homozygous and hemizygous transgenic mice express GFP in all over the body. The transgene is located in X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies. | |||||||||||||||||||||||||||||||
| Colony maintenance | Sibling Mating (Homozygote x Hemizygote) | |||||||||||||||||||||||||||||||
| Health Report |
BRC facility Health Report in the last year and a half
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| Gene Details | ||||||||||||||||||||||||||||||||
| Promoter | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||||||||||||||||||||||||||||||
| 1 | Symbol | GFP | ||||||||||||||||||||||||||||||
| Symbol name | Green Fluorescent Protein (Jellyfish) | |||||||||||||||||||||||||||||||
| Chromosome | X | |||||||||||||||||||||||||||||||
| Common name | No Data | |||||||||||||||||||||||||||||||
| Symbol description | No Data | |||||||||||||||||||||||||||||||
| References | FEBS Lett. 1997 May 5;407(3):313-9. Genomics. 2002 Dec;80(6):564-74. Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4039-44. Epub 2005 Mar 3. ‘Green mice’ as a source of ubiquitous green cells.
‘Green mice’ as a source of ubiquitous green cells. ‘Green mice’ as a source of ubiquitous green cells. |
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| Research applications | Fluorescent Proteins/lacZ System | |||||||||||||||||||||||||||||||
| Specific Term and Conditions | The following terms and conditions will be requested by the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Proc Natl Acad Sci USA.102(11), 4039-4044 (2005). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
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| Additional information | ||||||||||||||||||||||||||||||||
| 1 | GFP Transfer License (Japanese / English) Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B. Lab HP |
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| 2 | Genotyping protocol <PCR> | |||||||||||||||||||||||||||||||
| Depositor | Okabe, Masaru (Osaka university)  |
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| Strain Status / Availability (Expected delivery) |
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