RBRC00986, B6C3F1-Tg(CAG-EGFP)CX-FM50Osb

RBRC No. RBRC00986
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)CX-FM50Osb
Former Common name B6C3F1B6C3F1 TgN(act-EGFP)OsbCX-FM050
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.
Strain description Both homozygous and hemizygous transgenic mice express GFP in all over the body. The transgene is located in X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.
Colony maintenance Sibling Mating (Homozygote x Hemizygote)
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome X
Common name No Data
Symbol description No Data
References FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.
Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4039-44. Epub 2005 Mar 3.

‘Green mice’ as a source of ubiquitous green cells.
‘Green mice’ as a source of ubiquitous green cells.
‘Green mice’ as a source of ubiquitous green cells.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics, 80, 564-574 (2002). Proc Natl Acad Sci USA.102(11), 4039-4044 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months