Strain Information | |
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Image | |
BRC No. | RBRC00995 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Il5ra<tm1Ktk>/KtkRbrc |
Former Common name | C57BL/6 IL-5R alpha -/- |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | C57BL/6NCrSlc |
Appearance | |
Strain development | Developed by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6CrSlc. |
Strain description | L-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] |
References | Immunology, 102, 289-300 (2001). 11298827 Immunity, 4, 483-494 (1996). 8630733 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Il5rainterleukin 5 receptor, alpha6Il5ra<tm1Ktk>targeted mutation 1, Kiyoshi TakatsuCD125, CDw125, IL-5 receptor alpha chain, Il5r, IL-5Rα Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IL-5R α genomic DNA |
Research application | Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice. |
Depositor | Kiyoshi Takatsu (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kim HS, Lee MB, Lee D, Min KY, Koo J, Kim HW, Park YH, Kim SJ, Ikutani M, Takaki S, Kim YM, Choi WS. The regulatory B cell-mediated peripheral tolerance maintained by mast cell IL-5 suppresses oxazolone-induced contact hypersensitivity. Sci Adv 5(7) eaav8152(2019) 31328158 |