RBRC01000, B6.Cg-Fyn<tm1Yik>/TyagRbrc

RBRC No. RBRC01000
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Fyn<tm1Yik>/TyagRbrc
Former Common name B6.Cg-Fyn<tm1Yik>, Fyn Targeting mice
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6 and CBA mixed
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Takeshi Yagi, Tsukuba Life Science Center, RIKEN in 1993. The targeting vector containing lacZ-neo cassette was transferred into TT2 ES cells to replace the SH1, SH3 and tyrosine kinase regions of the Fyn gene. The mutant mice were crossed to C57BL/6.
Strain description Fyn gene knockout mice. Fyn proto-oncogene is a non-receptor type tyrosine kinase of Src family that is expressed in the central nervous system and suggested to be involved in the mechanism of neuronal signaling cascade. Homozygous mutant mice exhibit a defect in suckling behavior and abnormal hippocampus morphology. This strain is useful for elucidation of the role of Fyn tyrosine kinase.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Fyn
Symbol name Fyn proto-oncogene
Chromosome 10
Common name Src Kinase p59;
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 10
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name neo; neomycin;
Symbol description No Data
References Nature. 1993 Dec 23-30;366(6457):742-5.
Science 1997 Oct 24;278(5338):698-701.

A role for Fyn tyrosine kinase in the suckling behaviour of neonatal mice.
A role for Fyn tyrosine kinase in the suckling behaviour of neonatal mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 366, 742-745 (1993).
Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Yagi, Takeshi (National Institutes of Natural Sciences) Yagi, Takeshi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Inhibitory effects of parthenolide on antigen-induced microtubule formation and degranulation in mast cells. Int Immunopharmacol.8(6): 874-80 (2008).(18442792)
Miyata N, Gon Y, Nunomura S, Endo D, Yamashita K, Matsumoto K, Hashimoto S, Ra C.
FcepsilonRI beta-chain ITAM amplifies PI3K-signaling to ensure synergistic degranulation response via FcepsilonRI and adenosine receptors. Eur J Immunol.40(4): 1205-17 (2010).(20101614)
Nunomura S, Gon Y, Yoshimaru T, Kashiwakura J, Kawakami T, Ra C.