RBRC01046, B6.Cg-Atf2<tm1Sis>/Rbrc

RBRC No. RBRC01046
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.Cg-Atf2<tm1Sis>/Rbrc
Former Common name ATF2o Knock out (CRE-BP1)-B6
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Toshio Maekawa and Shunsuke Ishii, RIKEN Tsukuba Institute in 1999.
Strain description ATF2 (CRE-BP1) is a transcription factor of the ATF/CREB family. This protein has a zinc finger structure as a transactivation domain located at the amino terminus and a leucin zipper motif, and phosphorylated by the stress-activated protein kinase such as the Jun amino-terminal kinase and p38. ATF2 deficient mice die shortly after birth and display symptoms of severe respiratory distress with lungs filled with meconium. This strain is useful as an animal model for meconium aspiration syndrome (MAS).
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Atf2
Symbol name activating transcription factor 2
Chromosome 2
Common name ATF-2, CRE-BP, Creb2, D130078H02Rik, mXBP
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References J Biol Chem. 1999 Jun 18;274(25):17813-9.

Mouse ATF-2 null mutants display features of a severe type of meconium aspiration syndrome.
Research applications Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Maekawa, T., Bernier, F., Sato, M., Nomura, S., Singh, M., Inoue, Y., Tokunaga, T., Imai, H., Yokoyama, M., Reimold, A., Glimcher, L. H. & Ishii, S. Mouse ATF-2 null mutants display features of severe type of meconium aspiration syndrome. J. Biol. Chem., 274, 17813-17819 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
1. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
2. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Ishii, Shunsuke (RIKEN)
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months