RBRC01071, Floxed RBP-J

RBRC No. RBRC01071
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name Floxed RBP-J
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain Not Specified
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain description Rbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Homozygote x Homozygote. Homozygous mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Rbpj
Symbol name recombining binding protein suppressor of hairless (Drosophila)
Chromosome 5
Common name RBPf, RBP-JC, RBP-Jf, Rbpjflox, Rbp-Jflox, RBP-JkappaFl, RBP-Jkappaflox, RBPJkappaloxP, RBP-Jlox, RbpjloxP, Rbpsuhtm3Kyo
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References Int Immunol. 2002 Jun;14(6):637-45.

Notch signaling controls liver development by regulating biliary differentiation.
Notch signaling controls liver development by regulating biliary differentiation.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Notch signaling controls liver development by regulating biliary differentiation. Development.136(10): 1727-39 (2009).(19369401)
Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. Dev Biol.332(2): 339-50 (2009).(19501584)
Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature.466(7307): 765-8 (2010).(20639863)
Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
Notch pathway regulation of neural crest cell development in vivo. Dev Dyn.241(2): 376-89 (2012).(22275227)
Mead TJ, Yutzey KE.
Epithelial Cell-Intrinsic Notch Signaling Plays an Essential Role in the Maintenance of Gut Immune Homeostasis. J Immunol. (2012).(22279105)
Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
In vivo notch reactivation in differentiating cochlear hair cells induces sox2 and prox1 expression but does not disrupt hair cell maturation. Dev Dyn.241(4): 684-96 (2012).(22354878)
Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development. Proc Natl Acad Sci U S A. (2013).(23319657)
Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues. EMBO Rep.15(12): 1297-304 (2014).(25378482)
Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
A Genome-Wide Analysis Identifies a Notch-RBP-J kappa-IL-7R alpha Axis That Controls IL-17-Producing gamma delta T Cell Homeostasis in Mice. J Immunol. (2014).(25429074)
Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals. Elife6: (2017).(28395734)
Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.