Strain Data Sheet

RBRC01089

Strain Information

Image
BRC No.RBRC01089
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Nras<tm1Mok>
Former Common nameN-ras KO, B6-Nras KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionNras gene knockout mice. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesOncogene, 15, 1151-1159 (1997). 9294608
EMBO J. 21(5):1021-30 (2002). 11867530
Oncogene, 27, 2961-2968 (2008). 18059342

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Csde1cold shock domain containing E1, RNA binding3Csde1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrasneuroblastoma ras oncogene3Nras<tm1Mok>targeted mutation 1, Motoya KatsukiN-ras

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse N-ras genomic DNA, mouse Csde1 genomic DNA
Research applicationCancer Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 21, 1021-1030 (2002). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data