RBRC01099, ERAI mice

RBRC No. RBRC01099
Type TransgeneCartagena
Species Mus musculus
Strain name ERAI mice
Former Common name ER stress-activated indicator (ERAI)
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Takao Iwawaki and Masayuki Miura, RIKEN Brain Science Institute. The 3.6-kb fragment of pCAX-F-XBP1deltaDBD-venus was injected into the pronuclei of C57BL/6 fertilized eggs. Transgenic mice were backcrossed to C57BL/6JJcl.
Strain description ERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]. Tg mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol XBP1
Symbol name X-box binding protein 1 (human)
Chromosome UN
Common name D11Ertd39e, TREB-5, TREB5, TREB5, XBP-1
Symbol description No Data
2 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome UN
Common name Venus
Symbol description No Data
Promoter CMV-IE enhancer, chicken beta-actin promoter
3 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
References Nat Med. 2004 Jan;10(1):98-102. Epub 2003 Dec 14.

Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
Research applications Cell Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Med., 10, 98-102 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
During 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR, and to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA. If there is intent to use the material for profit purpose, the recipient shall first discuss its intentions with RIKEN. The recipient shall agree to acknowledge RIKEN in all publications with regard to the material.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Mar 2007
Genetic Background
3 Genotyping protocol <PCR>
Depositor Miura, Masayuki (The University of Tokyo) Miura, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Pseudomonas aeruginosa activates the unfolded protein response in airway epithelia: relevance for airway in inflammation. Pediatric Pulmonology, Supplement30: 260 (2007).
Braun, M., Tatreau, J., Fulcher, N., Wolfgang, M., Randell, S., O eal, W., and Ribeiro, C. M. P.
Endoplasmic reticulum stress regulator XBP-1 contributes to effector CD8+ T cell differentiation during acute infection. J Immunol.181(8): 5433-41 (2008).(18832700)
Kamimura D, Bevan MJ.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1. J Biol Chem.284(22): 14904-13 (2009).(19321437)
Martino ME, Olsen JC, Fulcher NB, Wolfgang MC, O’Neal WK, Ribeiro CM.
ER stress is Involved in T17M Rhodopsin-Induced Retinal Degeneration. Invest Ophthalmol Vis Sci. (2012).(22589437)
Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk MS.
Modulation of Angiogenesis by Genetic Manipulation of ATF4 in a Mouse Modulation of Angiogenesis by Genetic Manipulation. Invest Ophthalmol Vis Sci.54(9): 5995-6002 (2013).(23942974)
Wang X, Wang G, Kunte M, Shinde V, Gorbatyuk M.
Asna1/TRC40 controls beta cell function and ER homeostasis by ensuring retrograde transport. Diabetes (2015).(26438609)
Norlin S, Parekh VS, Naredi P, Edlund H.
Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa. PLoS One. (2016).(27144303)
Bhootada Y, Kotla P, Zolotukhin S, Gorbatyuk O, Bebok Z, Athar M, Gorbatyuk M.