RBRC01101, B6.129S2-Rbpj<tm1Kyo>/HonRbrc

RBRC No. RBRC01101
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129S2-Rbpj<tm1Kyo>/HonRbrc
Former Common name RBP-J knockout mouse
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain C57BL/6NCrSlc
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Honjo, Faculty of Medicine, Kyoto University in 1995.
Strain description RBP-J, recombining binding protein suppressor of hairless (Drosophila) which is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. RBP-J homozygous mutant mice show severe growth retardation at 8.5 days of gestation, incomplete turning of the body axis, microencephaly, abnormal placental development, anterior neuropore opening and defective somitogenesis at 9.5 days of gestation, and die before 10.5 days of gestation.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rbpj
Symbol name recombining binding protein suppressor of hairless (Drosophila)
Chromosome 5
Common name RBPjk-, RBP-Jkappa-
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References Development. 1995 Oct;121(10):3291-301.

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Development, 121, 3291-3301 (1995).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock. Dev Cell.13(2): 298-304 (2007).(17681139)
Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.