RBRC01120, B6.Cg-Agt<tm1Afu>/AfuRbrc

RBRC No. RBRC01120
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.Cg-Agt<tm1Afu>/AfuRbrc
Former Common name AGT-KO (BL/6)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Tanimoto K., University of Tsukuba in 2004. C57BL/6 background.
Strain description B6.B6CB-Agt<tm1Afu>. Homozygous mutant mice do not produce angiotensinogen in the liver, resulting in the complete loss of plasma immunoreactive angiotensin I. The systolic blood pressure of the homozygous mutant is lower than wild-type, demonstrates an indispensable role for the renin-angiotensin system in maintaining blood pressure.
Colony maintenance Sibling Mating (Heterozygote x Wild-type)
Homozygous mutant mice of C57BL/6 background show a low survivability. About 70 percent of homozygous mice
Health Report
Gene Details
Promoter No Data
1 Symbol Agt
Symbol name Angiotensinogen
Chromosome 8
Common name Aogen
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References J Biol Chem. 1994 Dec 16;269(50):31334-7.

Angiotensinogen-deficient mice with hypotension.
Research applications Cell Biology Research,
Hematological Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 269, 31334-31337 (1994).
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Fukamizu, Akiyoshi (University of Tsukuba) Fukamizu, Akiyoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months