RBRC01130, B6.129P2-Ugt8a<tm1Pop>/Rbrc

RBRC No. RBRC01130
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Ugt8a<tm1Pop>/Rbrc
Former Common name Ceramide galactosyltransferase (CGT)-deficient mice
H-2 Haplotype No Data
ES cell line BK4 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via BK4 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Brain Popko, University of North Carolina in 1996.
Strain description CGT-deficient mice fail to make galactolipid galactocerebroside and its sulfated derivative that are normal myelin constitutents. Mutant mice exhibit tremors, ataxia, progressive hindlib paralysis and vaculoe formation in ventral spinal cord due to abnormal myelin sheath.
B6.129P2-Ugt8a<tm1Pop>. Ceramide galactosyltransferase, CGT is a key enzyme in the biosynthesis of galactocerebroside, GalC. Homozygous mutant mice for CGT gene show tremor, ataxia progressive hind limb paralysis and extensiv vacuolation of the ventral spinal cord.
Colony maintenance Sibling Mating
Health Report
Gene Details
Promoter No Data
1 Symbol Ugt8
Symbol name UDP-glucuronosyltransferase 8
Chromosome 3
Common name Cgt
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name neo; neomycin;
Symbol description No Data
References Cell. 1996 Jul 26;86(2):209-19.
J Biol Chem. 2000 Jul 28;275(30):22623-6.
J Lipid Res. 2000 Aug;41(8):1237-43.
Tanpakushitsu Kakusan Koso 1998 Dec;43(16 Suppl):2318-24.
Tanpakushitsu Kakusan Koso. 2001 Jan;46(1):45-53.

Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.
Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.
Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.
Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.
Myelination in the absence of galactocerebroside and sulfatide: normal structure with abnormal function and regional instability.
Research applications Cell Biology Research,
Neurobiology Research
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Popko, Brian (The University of Chicago) Popko, Brian
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months