Strain Information | |
---|---|
Image | |
BRC No. | RBRC01130 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Ugt8a<tm1Pop>/Rbrc |
Former Common name | Ceramide galactosyltransferase (CGT)-deficient mice |
H-2 Haplotype | |
ES Cell line | BK4 [129P2/OlaHsd] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Brain Popko, University of North Carolina in 1996. |
Strain description | CGT-deficient mice fail to make galactolipid galactocerebroside and its sulfated derivative that are normal myelin constitutents. Mutant mice exhibit tremors, ataxia, progressive hindlib paralysis and vaculoe formation in ventral spinal cord due to abnormal myelin sheath.B6.129P2-Ugt8a<tm1Pop>. Ceramide galactosyltransferase, CGT is a key enzyme in the biosynthesis of galactocerebroside, GalC. Homozygous mutant mice for CGT gene show tremor, ataxia progressive hind limb paralysis and extensiv vacuolation of the ventral spinal cord. |
Colony maintenance | Sibling Mating |
References | J. Lipid Res., 41, 1237-1243 (2000). 10946011 蛋白質 核酸 酵素, 43,2318-2324 (1998). 9883654 Cell, 86, 209-219 (1996). 8706126 J. Biol. Chem., 275, 22623-22626 (2000). 10801776 蛋白質 核酸 酵素, 46, 45-54 (2001). 11193331 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3neo; neomycin; |
Ordering Information | |
---|---|
Donor DNA | mouse Ceramide galactosyltransferase (CGT) genomic DNA, E. coli neo |
Research application | Cell Biology Research Neurobiology Research |
Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
Depositor | Brian Popko (University of Chicago) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |