RBRC01145, ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc

RBRC No. RBRC01145
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc
Former Common name Mesp1-cre
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain ICR MCH
Appearance
1 Appearance Agouti or black
Genotype A/? or a/a B/B Mesp1-Cre c/+ C
2 Appearance albino
Genotype A/? or a/a B/B + c/+ C
Strain development Developed by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background.
Strain description Mesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal.
Colony maintenance Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal.
Health Report
Gene Details
Promoter No Data
1 Symbol Mesp1
Symbol name mesoderm posterior 1
Chromosome 7
Common name No Data
Symbol description No Data
Promoter Mesp1
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Development. 1999 Aug;126(15):3437-47.

Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development, 126, 3437-3447 (1999).
Additional information
1 Saga Lab HP
2 Mouse of the Month Feb 2006
Genetic Background
3 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency. Cardiovasc Res.79(3): 448-57 (2008).(18440989)
MacDonald ST, Bamforth SD, Chen CM, Farthing CR, Franklyn A, Broadbent C, Schneider JE, Saga Y, Lewandoski M, Bhattacharya S.
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell.3(1): 55-68 (2008).(18593559)
Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
Tissue-specific requirements for FGF8 during early inner ear development. Mech Dev.126(10): 873-81 (2009).(19619645)
Dominguez-Frutos, E., Vendrell, V., Alvarez, Y., Zelarayan, L.C., Lopez-Hernandez, I., Ros, M. and Schimmang, T.
Great vessel development requires biallelic expression of Chd7 and Tbx1 in pharyngeal ectoderm in mice. J Clin Invest.119(11): 510518 (2009).(19855134)
Randall V, McCue K, Roberts C, Kyriakopoulou V, Beddow S, Barrett AN, Vitelli F, Prescott K, Shaw-Smith C, Devriendt K, Bosman E, Steffes G, Steel KP, Simrick S, Basson MA, Illingworth E, Scambler PJ.
Lineage tracing of the endoderm during oral development. Dev Dyn. (2012).(22581563)
Rothova M, Thompson H, Lickert H, Tucker AS.
The origin of the stapes and relationship to the otic capsule and oval window. Dev Dyn.241(9): 1396-404 (2012).(22778034)
Thompson H, Ohazama A, Sharpe PT, Tucker AS.