RBRC01191, B6;129-Meis1<tm1Ngc>/Rbrc

RBRC No. RBRC01191
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Meis1<tm1Ngc>/Rbrc
Former Common name Meis1 Knockout mice
H-2 Haplotype No Data
ES cell line JR2448 [129S3/SvImJ]
Donor strain 129S3/SvImJ via JR2448 ES cell line
Background strain C57BL/6J
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Masami Fujita and Neal Copeland, National Cancer Institute in 1997. Donor strain is 129S3/SvlmJ via JR2448 ES cell line. This strain was maintained in C57BL/6J and 129S3/SvlmJ mixed background.
Strain description B6;129S3-Meis1<tm1Ngc>. Meis1 deficient mice exhibit abnormal retina, vasculature, hematopoietic system defects. Homozygous mutant mice die by embryonic day 14.5.
Colony maintenance Litter size is a little small. Embryonic lethality (Homozygous).
Genotyping by PCR.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Meis1
Symbol name myeloid ecotropic viral integration site 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References EMBO J. 2004 Jan 28;23(2):450-9. Epub 2004 Jan 8.

Hematopoietic, angiogenic and eye defects in Meis1 mutant animals.
Research applications Fluorescent Proteins/lacZ System,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 23, 450-459 (2004).
Additional information
1 The Cancer Institute of JFCR
2 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months