RBRC01195, B6.129S6-Grin2a<tm1Nak>/NakRbrc

RBRC No. RBRC01195
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129S6-Grin2a<tm1Nak>/NakRbrc
Former Common name NR2A KO, C57BL/6J-Grin2a tm
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEvTac via EK.CCE cell line
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C)
Strain development Developed by Drs. H. Kadotani and S. Nakanishi, Kyoto University in 1996.
Strain description B6.129S6-Grin2a<tm1Nak>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice are viable and fertile.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6J)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grin2a
Symbol name glutamate receptor, ionotropic, NMDA2A (epsilon 1)
Chromosome 16
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 16
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 1996 Dec 15;16(24):7859-67.

Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Journal of Neuroscience, 16, 7859-7867 (1996).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nakanishi, Shigetada (Kyoto University) Nakanishi, Shigetada
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36. Evid Based Complement Alternat Med.2012: 438460 (2012).(23118791)
Gao X, Qiao Y, Jia B, Jing X, Cheng B, Wen L, Tan Q, Zhou Y, Zhu B, Qiao H.