RBRC01198, 129.Cg-Tg(GOF18dPE/EGFP)48Ymat

RBRC No. RBRC01198
Type Transgene CongenicCartagena
Species Mus musculus
Strain name 129.Cg-Tg(GOF18dPE/EGFP)48Ymat
Former Common name 129;B6D2-Tg(GOF18δPE/EGFP)48, GOF18-delta PE-GFP.129
H-2 Haplotype No Data
Donor strain B6D2F1
Background strain 129/Sv (hemizygote x 129/Sv)
Appearance
1 Appearance White-bellied gray
Genotype No Data
Strain development Developed by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).
Strain description Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).
Colony maintenance Carrier x Noncarrier [or Crossing to 129<+Ter>/SvJcl]
Health Report No Data
Gene Details
Promoter Oct 3 promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Dev Growth Differ. 1999 Dec;41(6):675-84.

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Purpose of study should be clarified.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Ogura, Atsuo (RIKEN, BioResource Center) Ogura, Atsuo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Microinsemination with first-wave round spermatids from immature male mice. J Reprod Dev.50(1): 131-7 (2004).(15007210)
Miki H, Lee J, Inoue K, Ogonuki N, Noguchi Y, Mochida K, Kohda T, Nagashima H, Ishino F, Ogura A.
Bone marrow cells differentiate in cardiac cell lineages after infarction independently of cell fusion. Circ. Res.96: 127-137 (2005).(15569828)
Jan KAJSTURA, Marcello ROTA, Brian WHANG, Stefano CASCAPERA, Toru HOSODA, Claudia BEARZI, Daria NURZYNSKA, Hideko KASAHARA, Elias ZIAS, Massimiliano BONAFE, Bernardo NADAL-GINARD, Daniele TORELLA, Angelo NASCIMBENE, Federico QUAINI, Konrad URBANEK, Annarosa LERI, Piero ANVERSA