RBRC01230, B6;129-Foxh1<tm1Hmd>/HmdRbrc

RBRC No. RBRC01230
Type Targeted Mutation and Chromosomal Aberration and F2 HybridCartagena
Species Mus musculus
Strain name B6;129-Foxh1<tm1Hmd>/HmdRbrc
Former Common name B6;129-FoxH1flox/flox, FASTflox/flox, B6;129-FoxH1tm2Hh(B6;129-FoxH1flox/flox,FASTflox/flox)Knockout mice
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
2 Appearance white-bellied agouti
Genotype A/? B/B C/C
Strain development Developed by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. C57BL/6Cr and (129X1/SvJ x 129S1/Sv)F1-Kitl+ mixed background.
Strain description The transcription factor Foxh1 (horkhead box H1) gene mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 protein interacts with phospho-Smad2 to regulate specific downstream targets of these signals. Mutation in Foxh1 results in impaired orientation of anterior-posterior axis and various defects of anterior streak and node. The flox allele of this strain was generated by Flp-mediated deletion of the neo cassette located between frt sites from the original targeting vector which was designed to insert a combined loxP site and loxP-frt-neo-frt cassette. Conditional Foxh1 deficient mice can be generated by crossing with tissue-specific Cre mice. This strain is useful for the study of Foxh1 gene in vivo.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygote mutant mice are normal and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol FoxH1
Symbol name forkhead box H1
Chromosome 15
Common name FAST
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References Genes Dev. 2001 May 15;15(10):1242-56.

The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on
use of it from the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months