RBRC01253, B6;129S4-Gopc<tm1.1Tno>/Rbrc

RBRC No. RBRC01253
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Gopc<tm1.1Tno>/Rbrc
Former Common name GOPC Knockout mouse
H-2 Haplotype No Data
Donor strain 129S4/SvJae via J1 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Drs. Ryoji Yao and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. Genomic sequence containing 2, 2b, 3 and 4 of Gopc was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 bachground.
Strain description B6;129S4-Gopc<tm1.1Tno>. Gopc, golgi associtated PDZ and coiled-coil motif containing that predominantly localized at trans-Golgi region, propose that play a role in vesicle transport from the Golgi. The Gopc deficient male mice are infertile that spermatozoa showed complete lack of acrosomes. This knockout mice are proved to be powerful tools for understanding the mechanisms underlying spermatogenesism, and valuable animal mode for human globozoospermia.
Colony maintenance Sibling Mating (Heterozygote x Wild-type, Heterozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol Gopc
Symbol name golgi associated PDZ and coiled-coil motif containing protein
Chromosome 10
Common name 2210402P09Rik, GOPC1
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 10
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 10
Common name No Data
Symbol description No Data
5 Symbol poly A
Symbol name poly A
Chromosome 10
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11211-6. Epub 2002 Jul 30.

Lack of acrosome formation in mice lacking a Golgi protein, GOPC.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. USA, 99, 11211-11216 (2002).
It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.
The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Noda, Tetsuo (The CANCER INSTITUTE of the Japanese Foundation for Cancer Research) Noda, Tetsuo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months