RBRC01274, B6.Cg-Tg(MT1-Kit * VAL620ALA)1/Rbrc

RBRC No. RBRC01274
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(MT1-Kit*VAL620ALA)1/Rbrc
Former Common name mt-KITV620A Tg-1 BDF1 x B6
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
2 Appearance black with white spot
Genotype a/a B/B C/C Tg/+
Strain development Developed by Dr. Takahiro Kunisada, Gifu University School of Medicine in 2004. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. Transgenic mice were backcrossed to C57BL/6.
Strain description B6;D2-Tg(MT1-kit,VAL620ALA). Transgenic mouse expresses the Val 620 Ala mutated Kit gene. Mice carrying the transgene show white spots on one fourth of the body hair. This transgenic mice are useful for the study of Kit gene function.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl)
Hemizygous mice show white spots on one fourth of the body hair.
Reproductive performance is good.
Health Report No Data
Gene Details
Promoter mouse MT-I promoter, human growth hormone poly A, 5′ and 3′ flanking regions of mouse MT gene
1 Symbol Kit
Symbol name kit oncogene
Chromosome UN
Common name mouse c-kit cDNA (Val 620Ala )
Symbol description No Data
References J Invest Dermatol. 2006 May;126(5):1111-8.

Mice transgenic for Kit(V620A): recapitulation of piebaldism but not progressive depigmentation seen in humans with this mutation.
Research applications Cancer Research,
Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on
use of it from the DEPOSITOR. The RECIPIENT is requested to include the
DEPOSITOR as a co-author in the first publication resulted by the use of the
BIOLOGICAL RESOURCE provided within two years after the deposition to the
RIKEN BRC. After acceptance of the first paper the DEPOSITOR only requests
the RECIPIENT to cite the first paper by the DEPOSITOR in any publication.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kunisada, Takahiro (Gifu University, Graduate School of Medicine) Kunisada, Takahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months