RBRC01294, CAT-Rho-K DN/3-1

RBRC No. RBRC01294
Type TransgeneCartagena
Species Mus musculus
Strain name CAT-Rho-K DN/3-1
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain description These transgenic mice express a dominant-negative form for RhoA for Rho-kinase (Rho-K DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Copy number: line 3-1 (RBRC01294)_10 copies, line 5-18 (RBRC01298)_1 copy.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl] Tg mice are viable and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rock2
Symbol name Rho-associated coiled-coil containing protein kinase 2 (bovine)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurosci 2004 Apr 7;24(14):3480-8.

Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase.
Research applications Cre/loxP system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neursci., 24, 3480-3488 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Jun 2005
3 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Non-cell-autonomous roles for the planar cell polarity gene Vangl2 in development of the coronary circulation. Circ Res.102(5): 615-23 (2008).(18174466)
Phillips HM, Hildreth V, Peat JD, Murdoch JN, Kobayashi K, Chaudhry B, Henderson DJ.
Crucial Role of Rho-Kinase in Pressure Overload-Induced Right Ventricular Hypertrophy and Dysfunction in Mice. Arterioscler Thromb Vasc Biol. (2014).(24675663)
Ikeda S, Satoh K, Kikuchi N, Miyata S, Suzuki K, Omura J, Shimizu T, Kobayashi K, Kobayashi K, Fukumoto Y, Sakata Y, Shimokawa H.
Rho-Kinase Inhibition Ameliorates Metabolic Disorders through Activation of AMPK Pathway in Mice. PLoS One.9(11) e110446: e110446 (2014).(25365359)
Noda K, Nakajima S, Godo S, Saito H, Ikeda S, Shimizu T, Enkhjargal B, Fukumoto Y, Tsukita S, Yamada T, Katagiri H, Shimokawa H.
The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination with Cell Polarization During Ameloblast Differentiation. J. Bone Miner Res. (2016).(27218883)
Otsu K, Ida-Yonemochi H, Fujiwara N, Harada H.