RBRC01300, D2.Cg-Prkdc<scid>/Rbrc

RBRC No. RBRC01300
Type Spontaneous Mutation
Species Mus musculus
Strain name D2.Cg-Prkdc<scid>/Rbrc
Former Common name DBA/2-scid/+, DBA/2-scid mice
H-2 Haplotype No Data
Donor strain DBA/2CrSlc
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Mie University Graduate School of Medicine. The original scid mice were provided by Ohtura Niwa, Radiation Biology Center, Kyoto University (C57BL/6-scid p<un>). The mutant mice were backcrossed to DBA/2Cr and C57BL/6NCr (RBRC01300 and RBRC01346, respectively).
Strain description scid, severe combined immune deficiency mice. Homozygous mutant mice lack both functional T and B cells, resulting in severe combined immunodeficiency. DBA/2 background (RBRC01300), C57BL/6 background (RBRC01346). We do not carry out a detection of leaky mice by measuring serum Ig level (PCR genotying of scid mutation only).
Colony maintenance Homozygote x Homozygote [or Crossing to DBA/2CrSlc]
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Mar 3, 2008 2-検 L
Jan 7, 2008 2-検 L
Oct 29, 2007 2-検 L
Jul 2, 2007 2-検 L
May 8, 2007 2-検 L
May 7, 2007 2-検 L
Mar 5, 2007 2-検 L
Jan 9, 2007 2-検 L

Gene Details
Promoter No Data
1 Symbol Prkdc
Symbol name protein kinase, DNA activated, catalytic polypeptide
Chromosome 16
Common name No Data
Symbol description No Data
References Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2438-43.
Radiat Res. 2002 Jun;157(6):661-7.

Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.
Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for basic research, shall not use for commercial use.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kuribayashi, Kagemasa (Mie University) Kuribayashi, Kagemasa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months