RBRC01307, C.Cg-Hbegf<tm1.1Mek>/MekRbrc

RBRC No. RBRC01307
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Hbegf<tm1.1Mek>/MekRbrc
Former Common name HB-EGF Knockout mice(BALB) (HBdel#80/BALB)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain BALB/cCr
Background strain BALB/cCrSlc
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.
The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice.
Strain description Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mutant mice die by 3 weeks of age. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically.
Colony maintenance Backcross to BALB/c (Heterozygote x BALB/cCrSlc)
Most homozygous mutant mice were die by 3 weeks of age.
Health Report
Gene Details
Promoter No Data
1 Symbol Hbegf
Symbol name heparin-binding epidermal growth factor-like growth factor
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 18
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 18
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 18
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3221-6. Epub 2003 Mar 5.

Heparin-binding EGF-like growth factor and ErbB signaling is essential for heart function.
Research applications Cardiovascular Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Apr 2006
3 Genotyping protocol <PCR>
Depositor Mekada, Eisuke (Osaka University) Mekada, Eisuke
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months