RBRC01347, B6.Cg-Tg(CAG-floxed neo-DT)03Osb

RBRC No. RBRC01347
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(CAG-floxed neo-DT)03Osb
Former Common name REP-DT
H-2 Haplotype No Data
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaru Okabe, Genome Information Research Center, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain description CAG-DT conditional transgenic mice (REP-DT mice). The transgene consists of the CAG promoter and the diphtheria toxin A chain (DT) cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, DT is expressed. It has been reported that healthy but sterile mice due to a disruption of germ line cells are generated when the REP-DT mice were mated with testis specific cre mice (Prm1-cre). Other tissue/organ-specific expression patterns of the transgene are not clear (Caution: The gene expression pattern in transgenic mice can be different from it of the endogenous gene.)
Colony maintenance Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol DT
Symbol name diphtheria toxin A (DT-A)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.

Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :