RBRC01351, B6.129S-Grm2<tm1Nak>/NakRbrc

RBRC No. RBRC01351
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129S-Grm2<tm1Nak>/NakRbrc
Former Common name C57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO
H-2 Haplotype No Data
Donor strain 129S6/SvEvTac via EK.CCE ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times.
Strain description mGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grm2
Symbol name G protein-coupled receptor, family C, group 1, member B
Chromosome 9
Common name 4930441L02Rik, Grm2, mGluR2
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4170-5. Epub 2005 Mar 7.
Science. 1996 Aug 2;273(5275):645-7.

Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Enhanced cocaine responsiveness and impaired motor coordination in metabotropic glutamate receptor subtype 2 knockout mice.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nakanishi, Shigetada (Kyoto University) Nakanishi, Shigetada
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists. Neurosci Lett.493(3): 26542 (2011).(21276828)
Moreno JL, Holloway T, Albizu L, Sealfon SC, Gonz lez-Maeso J.
Decoding the Signaling of a GPCR Heteromeric Complex Reveals a Unifying Mechanism of Action of Antipsychotic Drugs. Cell.147(5): 1011-23 (2011).(22118459)
Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, Mackerell AD Jr, Brezina V, Sealfon SC, Filizola M, Gonz lez-Maeso J, Logothetis DE.
Identification of Three Residues Essential for 5-Hydroxytryptamine 2A-Metabotropic Glutamate 2 (5-HT2A{middle dot}mGlu2) Receptor Heteromerization and Its Psychoactive Behavioral Function. J Biol Chem.287(53): 44301-19 (2012).(23129762)
Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cu llar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, L pez-Gim nez JF, Meana JJ, Benson DL, Gonz lez-Maeso J.
Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice. Neurosci Lett. (2013).(23333599)
Moreno JL, Holloway T, Rayannavar V, Sealfon SC, Gonz lez-Maeso J.
Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia. Sci. Signal.9(410): ra5. (2016).(26758213)
Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J.
Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors. Mol Neurobiol. (2016).(27878759)
Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL.