RBRC01353, C57BL/6-Tg(grm6-lacZ)35Kyo- grm6 tm1Kyo

RBRC No. RBRC01353
Type Targeted Mutation and TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(grm6-lacZ)35Kyo- grm6tm1Kyo
Former Common name mGluR6-/-/lacZ+
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shigetada Nakanishi, Kyoto University Faculty of Medicine. Tg: the transgene was injected into the pronuclei of B6D2F1 fertilized eggs. KO: CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c> were used to generate the mutant mice. C57BL/6 semicongenic.
Strain description Double mutant mice with transgene and knockout allele. Grm6 (glutamate receptor, metabotropic 6) gene is suggested to be responsible for ON responses in both the rod and cone systems. The transgene is mGluR6 gene fused to the beta-galactosidase reporter gene. This strain is useful for investigating the promoter function of the cell-specific and developmentally regulated expression of mGluR6.
Colony maintenance Tg: Carrier x Carrier; KO: Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Grm6
Symbol name glutamate receptor, metabotropic 6
Chromosome 11
Common name mGluR6
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
Promoter mouse mGluR6 promoter
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
References Cell. 1995 Mar 10;80(5):757-65.
J Neurosci. 1997 May 1;17(9):3014-23.
J Neurosci. 1999 Apr 1;19(7):2568-79.

Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Research applications General Purpose,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 19, 2568-2579 (1999). J. Neurosci., 17, 3014-3023 (1997). Cell, 80, 757-765 (1995).
The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nakanishi, Shigetada (Kyoto University) Nakanishi, Shigetada
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months